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Species
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Gene ID
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Accession
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Source
|
Length
|
MW
|
Tag
|
|
Mouse
|
20304
|
P30882
|
E. coli
|
68aa
|
7.9kDa
|
－
|
|
About this Antibody
|
|
Name
|
Recombinant Murine RANTES/CCL5 (Recombinant Murine Regulation upon Activation Normal T cell Express Sequence/CCL5; rMuRANTES/CCL5)；Recombinant Mouse RANTES/CCL5 ; 重组小鼠正常T细胞表达和分泌因子/CCL5
|
|
Synonyms
|
RANTES; SISd; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES
|
|
Purity
|
>97% by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T lymphocytes is in a concentration range of 1.0-10ng/ml.
|
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
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|
Formulation
|
Lyophilized from a 0.2μm filtered concentrated solution in 30% Acetonitrile and 0.1% TFA.
|
|
Endotoxin
|
Less than 1EU/μg of rMuRANTES/CCL5 as determined by LAL method.
|
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
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|
Category
|
Chemokines
|
|
Background
|
Chemokine (C-C motif) ligand 5 (CCL5) also known as RANTES is classified as a chemotactic cytokine or chemokine. It is encoded by the CCL5 gene in humans. CCL5 is chemotactic for T cells, eosinophils, and basophils, and plays an active role in recruiting leukocytes into inflammatory sites which has been reported to be produced by renal tubular epithelium, synovial fibroblasts and selected tumor cells. In addition, it also induces the proliferation and activation of certain natural-killer cells to form CHAK cells. Recombinant murine CCL5, a single non-glycosylated polypeptide, contains 68 amino acids and it shares 84% and 100%a.a. sequence identity with human and rat CCL5.
|
|
Amino Acid Sequence
|
SPYGSDTTPC CFAYLSLALP RAHVKEYFYT SSKCSNLAVV FVTRRNRQVC ANPEKKWVQE YINYLEMS
|
包装清单:
|
产品编号
|
产品名称
|
包装
|
|
P6780-5μg
|
Recombinant Murine RANTES/CCL5
|
5μg
|
|
P6780-20μg
|
Recombinant Murine RANTES/CCL5
|
20μg
|
|
P6780-100μg
|
Recombinant Murine RANTES/CCL5
|
100μg
|
|
P6780-1mg
|
Recombinant Murine RANTES/CCL5
|
1mg
|
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
党沛 
