应用
用于血清、血浆或其他相关生物液体中25羟基维生素D3的测定。
For the determination of Universal 25-HVD3(25-Hydroxy Vitamin D3) concentrations in serum, plasma and other related biological fluids.
技术规格
| 工作原理 | 本试剂盒采用的是竞争法酶联免疫吸附检测技术（ELISA）。测定样品中25羟基维生素D3水平。向预先包被了25羟基维生素D3抗原的酶标孔中，加入标准品和样本，温育后，加入生物素标记的抗25羟基维生素D3抗体。再与HRP标记的链霉亲和素结合，形成免疫复合物，再经过温育和洗涤，去除未结合的酶，然后加入显色底物TMB，产生蓝色，并在酸的作用下转化成最终的黄色。最后，在450 nm处测定反应孔样品吸光度（OD）值，样本中的25羟基维生素D3浓度与OD值成反比，通过绘制标准曲线计算出样本中25羟基维生素D3的浓度。 |
| Purpose | This assay employs the Competitive enzyme immunoassay technique. Determine the level of Universal 25-HVD3(25-Hydroxy Vitamin D3) in the sample. Add standard and sample to the microplate that have been pre-coated with Universal 25-HVD3(25-Hydroxy Vitamin D3) antigen. After incubation, add Biotin-conjugated anti-Universal 25-HVD3(25-Hydroxy Vitamin D3) antibody. It is then combined with HRP-conjugated streptavidin to form an immune complex, then incubated and washed to remove unbound enzyme, and then added to the chromogenic substrate TMB to produce a blue color, and converted to the final yellow under the action of acid. Finally, the absorbance (OD) value was measured at 450 nm. The concentration of Universal 25-HVD3(25-Hydroxy Vitamin D3) in the sample was proportional to the OD value. The concentration of Universal 25-HVD3(25-Hydroxy Vitamin D3) in the sample can be calculated by drawing a standard curve. |
| 灵敏度 | 1.88 ng/mL |
| Sensitivity | 1.88 ng/mL |
| 检测范围 | 3.13-200 ng/mL |
| Examination range | 3.13-200 ng/mL |
| 特异性 | 可检测样本中的25羟基维生素D3，且与其类似物无明显交叉反应。 |
| Specificity | This kit recognizes Universal 25-HVD3(25-Hydroxy Vitamin D3) in samples. No significant cross-reactivity or interference between Universal 25-HVD3(25-Hydroxy Vitamin D3) and analogues was observed. |
| 重复性 | 三种不同浓度的样本在同一块板子上分别检测20次，以及在不同的板子上分别检测20次，板内和板间变异系数均小于10%。 |
| Repeatability | Three different concentrations of samples were detected 20 times on the same board and 20 times on different boards. Within the plate or between the plates, the coefficient of variation is <10%. |
| 回收率 | 分别往稀释不同倍数的5个样本中加入已知浓度的目标蛋白，做回收实验，得出回收率范围和平均回收率。 |
| Recovery | A known concentration of the target protein was added to 5 samples which were diluted to different multiples, and a recovery experiment was performed to obtain a recovery range and an average recovery rate. |
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