| 敏感性 | 对湿度敏感 |
| 存贮条件 | 储存温度-20°C |
| 应用 | Erythrocyte agglutination studies;Model system of how proteins recognize carbohydrates |
| 产品介绍 | Phaseolus vulgaris lectin (PHA-E) is isolated from red kidney beans and purified by affinity chromatography and ion-exchange chromatography. It is a tetrameric protein with a molecular weight of 128 kDa. The lectin recognizes and binds specifically to terminal galactose, N-acetylglucosamine and mannose residues of complex glycans on mammalian glycoproteins. Because of its strong erythrocyte agglutinating activity, it is also known as Erythroagglutinin (PHA-E). PHA-E is not blood group specific. Less than 5 μg/ml agglutinates human erythrocytes of type O and agglutination can be inhibited by certain oligosaccharides. PHA-E lectin is supplied as a white lyophilized powder from a buffer containing from 50 mM NH4HCO3. The purity of the lectin in determined by SDS-PAGE, which generate one single band at 34 kDa. |
| 备注 | The lectin may be reconstituted with 2 ml of deionized water before use. Spin the vial gently until full dissolution. Aggregation is thought to occur in the presence of high concentrations of 2-mercaptoethanol. The solution may be reconstituted in this buffer to desired working concentration. |
| 生化机理 | PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity;PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. |
| 别名 | 植物血球凝集素；红腰豆/菜豆凝集素;Lectin from Phaseolus vulgaris (red kidney bean);Phaseolus vulgaris agglutinin; PHA |
外源凝集素(菜豆)
产品编号：P140098
品牌：阿拉丁
产品特点：
| 货号 | 包装 | 规格 | 目录价 | 您的价格 | 库存 | 数量 | 购买 |
|---|---|---|---|---|---|---|---|
| P140098-5mg | 5mg | ¥2574.90 |
|
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