熔点 | 196-198°C |
敏感性 | 对光线敏感,对空气敏感,对热敏感 |
存贮条件 | 储存温度: -20°C,充氩保存 |
应用 | 碱性磷酸酶(alkaline phosphatase)的底物。5-溴-4-氯-3-吲哚磷酸(BCIP) 和 氯化硝基四氮唑蓝(NBT)用于比色法鉴定碱性磷酸酶标记分子. BCIP/NBT 底物系统用途非常广泛:包括 Northern Southern, Western blotting, 原位杂交(in situ hybridization)和免疫组织化学。BCIP 可以有两种盐的形式: 二钠盐型溶于水,对甲苯胺型溶于二甲基甲酰胺(DMF)。这些盐型可用于制备和NBT结合的储存溶液和反应缓冲液,构成碱性磷酸酶的底物溶液。 底物系统和碱性磷酸酶一起培养,产生一种不溶的带紫色的NBT双甲腊( diformazan )产物,容易被观察。 |
产品介绍 | For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indoly?l phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid. |
别名 | 5-溴-4-氯-3-吲哚基磷酸酯对甲苯胺盐;X-phosphate p-toluidine salt |
5-溴-4-氯-3-吲哚基磷酸酯对甲苯胺盐(BCIP)
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt (订货以英文为准)
编号:B113076
CAS号:6578-06-9
分子式:C8H6BRCLNO4P.C7H9N
分子量:433.62
产品名称 | 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt |
中文名称 | 5-溴-4-氯-3-吲哚基磷酸酯对甲苯胺盐(BCIP) |
CAS号 | 6578-06-9 |
分子式(M.F.) | C8H6BRCLNO4P.C7H9N |
分子量(M.W.) | 433.62 |
储存条件 | -20°C储存;充氩 |
标识符号 | https://www.aladdin-e.com/images/ghs/ghs07.jpg,https://www.aladdin-e.com/images/ghs/ghs08.jpg |
信号词 | Danger |
危害声明 | H315,H319,H334,H335 |
警示性声明 | P261,P305+P351+P338,P342+P311 |
个人防护设备 | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
RTECS | YN5075000 |
WGK德国 | 1 |
风险声明 (欧洲) | R36/37/38;R42 |
安全声明(欧洲) | S22;S24;S26;S36/37 |
危害码(欧洲) | Xn |
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