存贮条件 | storage temp. -20°C |
应用 | Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source. Used to determine glucose, fructose, mannose and ATP. |
产品介绍 | Hexokinase catalyzes the reaction:D-hexose + ATP → D-hexose 6-phosphate + ADPHexokinases have been isolated from the yeast cell in two distinct forms, designated P-I and P-II (Schulze et al. 1969). These are separate, noninterconvertible isozymes (Womack et al. 1973).Hexokinase is used to determine glucose, fructose, mannose and ATP. |
生化机理 | Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source. The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C):D-fructose KM: 0.33 mM;D-glucose KM: 0.12 mM;D-mannose KM: 0.05 mM. Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively.Molecular Weight: ~ 54 kDa (monomer);~110 kDa (dimer);Optimal pH: 7.5 to 9.0;Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm. Activators: Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds. Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose?, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate). |
别名 | (重组)己糖激酶 rHK;recombinant Hexokinase |
(重组)己糖激酶 rHK
recombinant Hexokinase (订货以英文为准)
编号:H196998
CAS号:9001-51-8
货号 | 品牌 | 包装 | 目录价 | 您的价格 | 库存 | 数量 | 购买 |
---|---|---|---|---|---|---|---|
H196998-1KU | 阿拉丁 | 1KU | ¥720.90 |
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产品名称 | recombinant Hexokinase |
中文名称 | (重组)己糖激酶 rHK |
CAS号 | 9001-51-8 |
储存条件 | -20°C储存 |
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